Prevalence, and virulence determination of Listeria monocytogenes strains isolated from clinical and non-clinical samples by multiplex polymerase chain reaction

AUTOR(ES)

Pournajaf, Abazar, Rajabnia, Ramazan, Sedighi, Mansour, Kassani, Aziz, Moqarabzadeh, Vahid, Lotfollahi, Lida, Ardebilli, Abdollah, Emadi, Behzad, Irajian, Gholamreza

FONTE

Rev. Soc. Bras. Med. Trop.

DATA DE PUBLICAÇÃO

2016-10

RESUMO

Abstract INTRODUCTION: This study aimed to determine the prevalence, and virulence factors of Listeria monocytogenes isolated from various samples by multiplex polymerase chain reaction (MPCR). METHODS: A total of 617 isolates were obtained and MPCR was employed for detection of the inlA, inlC, and inlJ genes. RESULTS: L. monocytogenes was detected in 46 (7.45%) of the 617 specimens. inlA, inlC, and inlJ were detected in 100%, 76.26%, and 71% isolates, respectively. CONCLUSIONS: This study validated MPCR in the analysis and rapid detection of L. monocytogenes. The role of the genes in pathogenesis of the strains can also be affirmed.

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