Preparation and separation of d(pT)10·n oligonucleotides
AUTOR(ES)
Raae, A.J.
RESUMO
A series of oligomers having the general formula d(pT)10·n, n varying from 2 to 20, has been prepared by enzymatic joining of d(pT)10, annealed on poly dA, employing T4 polynucleotide ligase. The oligomers could be separated on 8 or 12% polyacrylamide gels. Such oligomers may prove useful as molecular weight markers and as initiators for various polymerases.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342847Documentos Relacionados
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