Preformed mRNA in Cotyledons of Ungerminated Seeds of Cicer arietinum L. 1

AUTOR(ES)

Matilla, Angel

RESUMO

Polyadenylated RNA was isolated from total RNA extracted from cotyledons of ungerminated or 18-hour-germinated chick-pea seeds by affinity chromatography on oligo(dT)-cellulose. Both poly(A)-containing RNA fractions exhibited a template activity when assayed in two cell-free translation systems, wheat germ extracts, and nuclease-treated reticulocyte lysates. Translation of preformed mRNA from cotyledons of dry seeds was completely abolished in the presence of several inhibitors of polypeptide chain initiation and also in the presence of the two “cap” analogues m7 GTP and m7 GMP. The patterns of polypeptides synthesized by translation of poly(A)-containing RNAs from cotyledons of ungerminated or 18-hour-germinated seeds, in the wheat germ system, analyzed by electrophoresis and autoradiography, were similar but not identical. It is concluded that cotyledons of dry Cicer arietinum L. seeds contain preformed mRNA.

Documentos Relacionados

• Purification and characterization of trypsin inhibitor from Cicer arietinum L. and its efficacy against Helicoverpa armigera
• Purification of trypsin inhibitor from seeds of Cicer arietinum (L.) and its insecticidal potential against Helicoverpa armigera (Hübner)
• Light regulation of specific mRNA species in Lemna gibba L. G-3
• Appearance of an Alternate Pathway Cyanide-resistant during Germination of Seeds of Cicer arietinum
• Senescence-Specific Increase in Cytosolic Glutamine Synthetase and Its mRNA in Radish Cotyledons 1