Prediction of major histocompatibility complex binding regions of protein antigens by sequence pattern analysis.
AUTOR(ES)
Sette, A
RESUMO
We have previously experimentally analyzed the structural requirements for interaction between peptide antigens and mouse major histocompatibility complex (MHC) molecules of the d haplotype. We describe here two procedures devised to predict specifically the capacity of peptide molecules to interact with these MHC class II molecules (IAd and IEd). The accuracy of these procedures has been tested on a large panel of synthetic peptides of eukaryotic, prokaryotic, and viral origin, and also on a set of overlapping peptides encompassing the entire staphylococcal nuclease molecule. For both sets of peptides, IAd and IEd binding was successfully predicted in approximately 75% of the cases. This suggests that definition of such sequence "motifs" could be of general use in predicting potentially immunogenic peptide regions within proteins.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=287118Documentos Relacionados
- The Genomic Sequence and Comparative Analysis of the Rat Major Histocompatibility Complex
- Isolation of carp genes encoding major histocompatibility complex antigens.
- A program for prediction of protein secondary structure from nucleotide sequence data: application to histocompatibility antigens.
- Simultaneous identification of campylobacters and prediction of quinolone resistance by comparative sequence analysis.
- Cloning and sequence analysis of the human major histocompatibility complex gene DC-3 beta.