Predicting the reactivity of proteins from their sequence alone: Kazal family of protein inhibitors of serine proteinases
AUTOR(ES)
Lu, Stephen M.
FONTE
The National Academy of Sciences
RESUMO
An additivity-based sequence to reactivity algorithm for the interaction of members of the Kazal family of protein inhibitors with six selected serine proteinases is described. Ten consensus variable contact positions in the inhibitor were identified, and the 19 possible variants at each of these positions were expressed. The free energies of interaction of these variants and the wild type were measured. For an additive system, this data set allows for the calculation of all possible sequences, subject to some restrictions. The algorithm was extensively tested. It is exceptionally fast so that all possible sequences can be predicted. The strongest, the most specific possible, and the least specific inhibitors were designed, and an evolutionary problem was solved.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=29270Documentos Relacionados
- Kazal-type serine proteinase inhibitors in the midgut of Phlebotomus papatasi
- Specific inhibition of mature fungal serine proteinases and metalloproteinases by their propeptides.
- Surface area is best estimated from weight alone: pocket calculators and nomograms are unnecessary.
- CARING FOR YOUR CHILDREN ALONE: THE FEMININE PERSPECTIVE OF THE MONOPARENTAL FAMILY
- Not on the face alone: perception of contextualized face expressions in Huntington's disease