Potential, pH, and arachidonate gate hydrogen ion currents in human neutrophils.
AUTOR(ES)
DeCoursey, T E
RESUMO
Indirect evidence indicates that a proton-selective conductance is activated during the respiratory burst in neutrophils. A voltage- and time-dependent H(+)-selective conductance, gH, in human neutrophils is demonstrated here directly by the whole-cell patch-clamp technique. The gH is extremely low at large negative potentials, increases slowly upon membrane depolarization, and does not inactivate. It is enhanced at high external pH or low internal pH and is inhibited by Cd2+ and Zn2+. Arachidonic acid, which plays a pivotal role in inflammatory reactions, amplifies the gH. The properties of the gH described here are compatible with its activation during the respiratory burst in stimulated neutrophils, in which it may facilitate sustained superoxide anion release by dissipating metabolically generated acid.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1225885Documentos Relacionados
- Escherichia coli intracellular pH, membrane potential, and cell growth.
- Alkalinizing the intralysosomal pH inhibits degranulation of human neutrophils.
- Intracellular pH modulates the generation of superoxide radicals by human neutrophils.
- Proton currents in human granulocytes: regulation by membrane potential and intracellular pH.
- Simple slide-rule conversion between hydrogen ion concentration and pH values.
