Potencial biotecnológico de fungos isolados do molusco Pugilina morio / Biotechnological potential of fungi isolated from shellfish Pugilina morio

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

24/02/2011

RESUMO

This study aimed to investigate the biotechnological potential of fungi isolated from mollusk Pugilina morio. The development work began with the collection of mollusk P. morio in Barra do Ceará, a beach situated in Fortaleza-CE. Of the specimens collected were isolated three filamentous fungi, known as PM3, PM6 and PM9. The isolates were identified by molecular analysis and classified as family Bionectriaceae, Aspergillus aculeatus and Penicillium sp, respectively. Subsequently we carried out the development of bioprocesses for obtaining metabolite with potential antitumor, antimicrobial, and enzymatic, focusing on classes of enzymes alcohol dehydrogenases (ADHs) and lipases. As a result of the tests antitumor of the eighteen extracts tested, six were active, inhibiting more than 75% of cell growth of at least two of the human tumor cell lines tested. Of the six active extracts, two from Aspergillus aculeatus and Penicillium sp grown in peptone-dextrose and dextrose- Czapeck, respectively, are more promising showing significant cytotoxic activity not related to damage to the plasma membrane. As a result of the antimicrobial activity, among the tested species, Aspergillus aculeatus showed moderate antagonistic activity against the phytopathogens Colletotrichum mursae and Fusarium oxysporum, with percentual mycelial growth inhibition values of 51 and 52% respectively while the Penicillium sp exhibited high antagonistic activity against Staphylococcus aureus ATCC 25923 with average inhibition zones of 13 mm. As a result of the enzimatic activities, the isolated Bionectriacea family proved active only for the ADHs activity and had moderate to high enantioselectivity, preferentially producing (S)-1-phenylethanol, enantiomeric excess (ee) ranging from 68 to 97%. Aspergillus aculeatus was shown to be active for both enzyme activities and showed low to moderate enantioselectivity producing (S) and (R)-1-phenylethanol with ee ranging from 3 to 25% and 39 to 52% respectively. Penicillium sp was also active in both assays and showed low and high enantioselectivities preferentially producing (S)-1-fenietanol with ee ranging from 31 to 95%. The enzymatic conversions were low with values ranging from 1 to 68%. In conclusion, the fungus Bionectriaceae family showed only ADHs activity positive, and promising for synthesis of chiral alcohols configuration S. Aspergillus aculeatus showed antitumor, antifungal, ADHs and lipase activities positive and promising for synthesis of chiral alcohols configurations R and S. Penicillium sp showed antitumor, antibacterial, ADHs and lipase activities positive, and promising for synthesis of chiral alcohols configuration S.

ASSUNTO(S)

pugilina morio fungos filamentosos atividade antitumoral atividade antimicrobiana atividades enzimáticas (adhs e lipases) biocatálise quimica pugilina morio filamentous fungi antitumor activity antimicrobial activity enzymatic activities (adhs and lipase) biocatalisys

ACESSO AO ARTIGO

Documentos Relacionados