Portal and periportal fibrosis in experimental extra-hepatic biliary obstruction in young and adult rats: contribution to biliary atresia study / "Fibrose portal e periportal na obstrução extra-hepática experimental em ratos jovens e adultos: contribuição para o estudo da atresia das vias biliares"

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

Biliary atresia is an hepatic disease of infancy. Etiology is unknown, and diagnosis is made by liver biopsy, with ductular proliferation being the main histological feature. Bile duct ligation in rats is an useful experimental model of biliary obstruction. The aim of this study of extra-hepatic cholestasis was analyse hepatic histological alterations in young rats compared to adult animals. The responses were studied by semiquantification of ductular proliferation and inflammatory infiltrated by HE stain; quantification of portal and periportal fibrosis with the sirius-red stain; semiquantification of the expression of desmin and a-smooth muscle actin by the hepatic stellated cells and myofibroblasts. In young animals, despite the very slow response of ductular proliferation and inflammation observed with HE, there were significantly more fibrosis and expression of desmin than in adult group

ASSUNTO(S)

ratos wistar fibrose/patologia histologia comparada imunohistoquÍmica atresia biliar disease models animals obstruÇÃo das vias biliares extra-hepÁticas/patologia cholestasis extrahepatic/pathology fibrosis/pathology immunohistochemistry histology comparative modelos animais de doenÇas biliary atresia rats wistar

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