Polymerase chain reaction assay of parvovirus B19 DNA in clinical specimens.
AUTOR(ES)
Clewley, J P
RESUMO
The polymerase chain reaction (PCR) was used to detect parvovirus B19 DNA in a panel of sera from individuals recently infected with B19, as demonstrated by the presence of anti-B19 immunoglobulin M. Of 95 serum samples, 60 (63%) were found positive by PCR, whereas only 1 was also found positive by dot hybridization. In a control panel of 100 serum samples from individuals with other infections, only 1 serum sample was found positive by PCR, and this was also found positive by dot hybridization. This was probably just a fortuitous discovery of viremia. Placental tissues from women (n = 89) who had proven B19 infections in pregnancy but who gave birth to healthy infants at term were also tested. A total of 74 (83%) were found positive for B19 DNA by PCR. The high rate of detection by PCR probably represents "decay" of viral DNA after the peak of viremia and is not a clinically significant phenomenon.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=267101Documentos Relacionados
- Dot blot hybridization assay of B19 virus DNA in clinical specimens.
- Detection of human parvovirus B19 DNA by using the polymerase chain reaction.
- Detection of parvovirus B19 in donated blood: a model system for screening by polymerase chain reaction.
- Characterization of a nested polymerase chain reaction assay for detection of parvovirus B19.
- Rapid screening for B19 parvovirus DNA in clinical specimens with a digoxigenin-labeled DNA hybridization probe.
