Polyamine requirement for efficient translation of amber codons in vivo.
AUTOR(ES)
Tabor, H
RESUMO
Multiplication of several amber mutants of bacteriophage T7 was decreased in two polyamine-deficient mutants of Escherichia coli K-12 carrying amber suppressors, relative to the multiplication of wild type bacteriophage T7 in the same hosts. In contrast the same T7 amber bacteriophages multiplied well in these strains when supplemented with polyamines. The requirement for polyamines for optimal translation of amber codons in vivo was confirmed by showing that infection of polyamine-depleted E. coli with bacteriophage T7 carrying an amber mutation in gene 1 resulted in an increased accumulation of the amber fragment of the gene 1 protein and a decreased accumulation of the full-length gene 1 protein compared with infection of an amine-supplemented culture. These results indicate that one important function of polyamines in vivo is concerned with protein translation and the protein-synthesizing ribosomal complex.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=347283Documentos Relacionados
- Requirement for 7-methylguanosine in translation of globin mRNA in vivo.
- The Schizosaccharomyces pombe sup3-i suppressor recognizes ochre, but not amber codons in vitro and in vivo.
- General requirement for RNA polymerase II holoenzymes in vivo.
- Encephalomyocarditis virus RNA. II. Polyadenylic acid requirement for efficient translation.
- Tetracycline-Regulated Suppression of Amber Codons in Mammalian Cells