Polar targeting of Shigella virulence factor IcsA in Enterobacteriacae and Vibrio
AUTOR(ES)
Charles, Macarthur
FONTE
National Academy of Sciences
RESUMO
Asymmetric localization is key to the proper function of certain prokaryotic proteins important to virulence, chemotaxis, cell division, development, motility, and adhesion. Shigella IcsA is localized to the old pole of the bacterium, where it mediates assembly of an actin tail inside infected mammalian cells. IcsA (VirG) is essential to Shigella intracellular motility and virulence. We used translational fusions between portions of IcsA and the green fluorescent protein (GFP) to determine the regions of IcsA that are necessary and sufficient for its targeting to the bacterial old pole. An IcsA-GFP fusion that lacks a signal peptide localized to the old pole, indicating that signal peptide-mediated secretion is not required for polar localization. Two regions within IcsA were required for localization of an IcsA-GFP fusion to the old pole. Further characterization of these regions indicated that amino acids 1–104 and 507–620 were each independently sufficient for polar localization. Finally, when expressed in Escherichia coli, Salmonella typhimurium, Yersinia pseudotuberculosis, and Vibrio cholerae, each of the two targeting regions localized to the pole, indicating that the mechanism of polar targeting used by IcsA is present generally among Enterobacteriacae and Vibrio.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=55545Documentos Relacionados
- Establishment of Unipolar Localization of IcsA in Shigella flexneri 2a Is Not Dependent on Virulence Plasmid Determinants
- Periplasmic Transit and Disulfide Bond Formation of the Autotransported Shigella Protein IcsA
- Functional Analysis of a Rickettsial OmpA Homology Domain of Shigella flexneri IcsA
- Shigella flexneri surface protein IcsA is sufficient to direct actin-based motility.
- Shigella flexneri DegP Facilitates IcsA Surface Expression and Is Required for Efficient Intercellular Spread