Plasmid-mediated formaldehyde resistance in Escherichia coli: characterization of resistance gene.
AUTOR(ES)
Kümmerle, N
RESUMO
The formaldehyde resistance mechanisms in the formaldehyde-resistant strain Escherichia coli VU3695 were investigated. A large (4.6-kb) plasmid DNA fragment encompassing the formaldehyde resistance gene was sequenced. A single 1,107-bp open reading frame encoding a glutathione- and NAD-dependent formaldehyde dehydrogenase was identified and sequenced, and the enzyme was expressed in an in vitro assay and purified. Amino acid sequence homology studies showed 62.4 to 63.2% identity with class III alcohol dehydrogenases isolated from horse, human, and rat livers. We demonstrated that the resistance mechanism in the formaldehyde-resistant strain E. coli VU3695 and in other formaldehyde-resistant members of the family Enterobacteriaceae is based on the enzymatic degradation of formaldehyde by a formaldehyde dehydrogenase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=163518Documentos Relacionados
- Energetics of plasmid-mediated arsenate resistance in Escherichia coli.
- Emergence of Plasmid-Mediated Quinolone Resistance in Escherichia coli in Europe
- Plasmid-mediated high-level resistance to erythromycin in Escherichia coli.
- Effects of Halides on Plasmid-Mediated Silver Resistance in Escherichia coli
- Plasmid-Mediated Quinolone Resistance in Clinical Isolates of Escherichia coli from Shanghai, China