PKA and MAPK phosphorylation of Prf1 allows promoter discrimination in Ustilago maydis
AUTOR(ES)
Kaffarnik, Florian
FONTE
Oxford University Press
RESUMO
Mating in Ustilago maydis requires cross-talk between cAMP and mitogen-activated protein kinase (MAPK) signalling. During this process, pheromone response factor 1 (Prf1) activates transcription of a and b mating type genes by binding to pheromone response elements (PREs) located in regulatory regions of these genes. Here, we show that PREs are also necessary and sufficient to mediate cAMP-induced gene expression. Prf1 interacts with cAMP-dependent protein kinase A (PKA) Adr1 as well as MAPK Kpp2 in vivo, and its central phosphorylation sites that are functionally important are modified by the respective kinases in vitro. PKA sites in Prf1 are essential for induced expression of a and b mating type genes. In contrast, MAPK sites are not required for pheromone-induced expression of a genes but are crucial for pheromone-responsive b gene expression. This illustrates how a single transcription factor can integrate signals from two pathways and how its phosphorylation status can determine different transcriptional responses.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=275411Documentos Relacionados
- The High-Mobility-Group Domain Transcription Factor Rop1 Is a Direct Regulator of prf1 in Ustilago maydis
- Environmental signals controlling sexual development of the corn Smut fungus Ustilago maydis through the transcriptional regulator Prf1.
- The induction of sexual development and virulence in the smut fungus Ustilago maydis depends on Crk1, a novel MAPK protein
- Perforin gene PRF1 c.900C> T polymorphism and HIV-1 vertical transmission
- Cloning and disruption of Ustilago maydis genes.