PIASy, a nuclear matrix–associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies
AUTOR(ES)
Sachdev, Shrikesh
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
The Wnt-responsive transcription factor LEF1 can activate transcription in association with β-catenin and repress transcription in association with Groucho. In search of additional regulatory mechanisms of LEF1 function, we identified the protein inhibitor of activated STAT, PIASy, as a novel interaction partner of LEF1. Coexpression of PIASy with LEF1 results in potent repression of LEF1 activity and in covalent modification of LEF1 with SUMO. PIASy markedly stimulates the sumoylation of LEF1 and multiple other proteins in vivo and functions as a SUMO E3 ligase for LEF1 in a reconstituted system in vitro. Moreover, PIASy binds to nuclear matrix–associated DNA sequences and targets LEF1 to nuclear bodies, suggesting that PIASy-mediated subnuclear sequestration accounts for the repression of LEF1 activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=312834Documentos Relacionados
- The retinoblastoma gene product is a cell cycle-dependent, nuclear matrix-associated protein.
- Intranuclear targeting of AML/CBFα regulatory factors to nuclear matrix-associated transcriptional domains
- Change in the expression of a nuclear matrix-associated protein is correlated with cellular transformation.
- RGS12TS-S Localizes at Nuclear Matrix-Associated Subnuclear Structures and Represses Transcription: Structural Requirements for Subnuclear Targeting and Transcriptional Repression
- Extracellular Matrix-Associated Protein Sc1 Is Not Essential for Mouse Development