Physicochemical Study of the Interaction between Gold Nanoparticles and Lipase from Candida sp. (CALB): Insights into the Nano-Bio Interface
AUTOR(ES)
Barros, Heloise R. de
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
21/10/2019
RESUMO
The surface interactions between gold nanoparticles (AuNPs) and lipase from Candida antarctica fraction B (CALB) were investigated at macromolecular and colloidal length-scales. In order to elucidate the reciprocal effect of the interactions on the individual component’s properties, CALB was either added during the synthesis of AuNPs or added to pre-synthesized AuNPs. In both cases, it was observed that the AuNPs are spherical and stable and, by fluorescence and circular dichroism spectroscopies, changes were observed in the secondary and tertiary structure of CALB, that were dependent on the AuNPs concentration. Nevertheless, the catalytic activity of CALB was maintained, although at a lower percentage (≥ 80%), thus new bio-functionalities were inserted into AuNPs upon interaction with CALB. Using simple and straightforward approaches and state-of-the-art techniques important knowledge about CALB/AuNPs bioconjugates was gained, thus contributing to the development of new nano-biomaterials and for the safe use of AuNPs in biomedical applications.
Documentos Relacionados
- DNA for nano-bio scale computation of chemical formalisms using Higher Order Logic (HOL) and analysis using an interdisciplinary approach
- Nano-assembled supramolecular films from chitosan-stabilized gold nanoparticles and cobalt(II) phthalocyanine
- Biosorption Potential of the Aspergillus sp. and Insights into Secondary Metabolism in the Presence of Copper and Lead
- Cloning and characterization of newly isolated lipase from Enterobacter sp. Bn12
- Characterization of a novel lipase from Bacillus sp. isolated from tannery wastes