Photosynthetic Enhancement Studied in Intact Spinach Chloroplasts 1

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RESUMO

The Emerson enhancement effect was evaluated in the intact spinach (Spinacia oleracea var. Long Standing Bloomsdale) chloroplast by monitoring the uptake of 14CO2 during illumination by 640 nm and 720 nm lights. Low levels (about 10 μm) of fructose 1,6-diphosphate, ribose 5-phosphate, and glycerate 3-phosphate stimulated the rate of photosynthesis and abolished enhancement values observed in their absence. Concentrations of the two sugar phosphates at levels of 1 mm responded similarly. In contrast, 1 mm glycerate 3-phosphate inhibited the rate of photosynthesis and increased enhancement. The exchange of glycerate 3-phosphate for glyceraldehyde 3-phosphate was speculated to be a factor underlying the decrease in photosynthesis and the increase in enhancement. Glucose 6-phosphate, NADPH, and l-malate did not influence photosynthesis or enhancement.

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