Photoperiodic Control of Gibberellin Metabolism in Spinach 1

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RESUMO

[3H]GA20 applied to spinach plants (Spinacia oleracea L.) was metabolized to several products. Two of these were identified by combined gasliquid chromatography-radio counting as [3H]GA29 and [3H]3-epi-GA1. Inasmuch as both GA20 and GA29 are endogenous gibberellins in spinach (Metzger, Zeevaart 1980 Plant Physiol 65: 623-626), it was concluded that the conversion of GA20 to GA29 is a natural process. However, 3-epi-GA1 was not detected in extracts of spinach shoots analyzed by combined gas chromatography-mass spectrometry. This indicates that the conversion of exogenous [3H]GA20 to [3H]3-epi-GA1 may be an artifact.

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