Pesquisa de oocistos de Cryptosporidium SPP e Salmonella SPP em amostras de águas de esgoto e águas de córrego da cidade de São Paulo. / Detection of Cryptosporidium spp oocysts and Salmonella spp in sewage and creek water from São Paulo city.
AUTOR(ES)
Eveline Wilma Coutinho Farias
DATA DE PUBLICAÇÃO
2000
RESUMO
A lot of studies have shown the value on researching in sewage several pathogenic microorganisms to determine possible enteropathogens that are present in communities and the risks for public health. The aims of this study were to determine the occurrence and the levels of Cryptosporidium spp and Salmonella spp in sewage and wastewater in São Paulo since there is no recent studies of this pathogens in sewage of this city. A total of twenty four samples were collected weekly from July to December of 1998 in an effluent sewage station (12) and a river (12) that receives a domestic discharge of São Paulo city. The detection of Cryptosporidium oocysts was made using two procedures for concentration by flocculation (Vesey et alii, 1993) and by membrane filtration (Aldom &Chagla, 1995), and oocysts were identified by direct immunofluorescence assay and the presence was confirmed by phase contrast microscopy. Salmonella isolation was made using membrane filtration method (Martins, 1979). The results showed the occurrence of Cryptosporidium sp and Salmonella spp in 100% of samples. A total of 221 strains of Salmonella were isolated and among the prevalent serotypes were detected S. panama, S. agona, S.infantis, S.hadar, S.onakan e S.braenderup. The presence of Salmonella and Cryptosporidium in environmental samples studied in São Paulo can represent a possible source for dissemination of these pathogens in aquatic evironment and community.
ASSUNTO(S)
sewage creek water poluição fecal salmonella cryptosporidium water quality qualidade da água poluted sewage indicadores padrão microbiológico salmolella águas de esgoto
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