Persistent Herpes Simplex Virus Infection In Vitro with Cycles of Cell Destruction and Regrowth
AUTOR(ES)
Hampar, Berge
RESUMO
Hampar, Berge (National Institute of Dental Research, Bethesda, Md.), and Mary Lou Copeland. Persistent herpes simplex virus infection in vitro with cycles of cell destruction and regrowth. J. Bacteriol. 90:205–212. 1965.—The susceptibility of two Chinese hamster cell lines to herpes simplex virus (HSV) was studied from the time of their initiation through successive subcultures. The cells' susceptibility to the cytocidal effects of HSV decreased as the number of cell passages increased. During the early cell passages, the decrease in cell susceptibility to HSV was characterized by an increased time after infection for complete cell destruction to occur, with a concomitant increase in the period when virus could be recovered from supernatant fluids. This was followed by a number of cell passages during which persistent HSV infections were established. The persistent infections were characterized by (i) cycles of virus synthesis and cell destruction followed by regrowth of the cells, (ii) initiation and maintenance under conditions optimal for cell growth in the absence of antibody, (iii) the cells' ability to be passaged while still maintaining their cycling patterns, (iv) a relationship between virus synthesis and cell proliferation, and (v) inability of long-term treatment with antibody to “cure” the persistent infections. The unique characteristics of this HSV infection were compared with other persistent in vitro viral infections.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=315614Documentos Relacionados
- Persistent Cyclic Herpes Simplex Virus Infection In Vitro III. Asynchrony in the Progression of Infection and Cell Regrowth
- Persistent Cyclic Herpes Simplex Virus Infection In Vitro II. Localization of Virus, Degree of Cell Destruction, and Mechanisms of Virus Transmission
- Regulation of persistent infection with herpes simplex virus in vitro by hydrocortisone.
- Persistent infection of human lymphoid and myeloid cell lines with herpes simplex virus.
- Persistent Cyclic Herpes Simplex Virus Infection In Vitro IV. Changes in the Severity of the Infections in the Presence of Antibody