Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus
AUTOR(ES)
Bittencourt-Cunha, Paula Rego, Silva-Cardoso, Livia, Oliveira, Giselle Almeida de, Silva, Jose Roberto da, Silveira, Alan Barbosa da, Kluck, George Eduardo Gabriel, Souza-Lima, Michele, Gondim, Katia Calp, Dansa-Petretsky, Marilvia, Silva, Carlos Peres, Masuda, Hatisaburo, Silva Neto, Mario Alberto Cardoso da, Atella, Georgia Correa
FONTE
Mem. Inst. Oswaldo Cruz
DATA DE PUBLICAÇÃO
2013-06
RESUMO
In this study, we describe the fate of fatty acids that are incorporated from the lumen by the posterior midgut epithelium of Rhodnius prolixus and the biosynthesis of lipids. We also demonstrate that neutral lipids (NL) are transferred to the haemolymphatic lipophorin (Lp) and that phospholipids remain in the tissue in which they are organised into perimicrovillar membranes (PMMs). 3H-palmitic acid added at the luminal side of isolated midguts of R. prolixus females was readily absorbed and was used to synthesise phospholipids (80%) and NL (20%). The highest incorporation of 3H-palmitic acid was on the first day after a blood meal. The amounts of diacylglycerol (DG) and triacylglycerol synthesised by the tissue decreased in the presence of Lp in the incubation medium. The metabolic fates of 3H-lipids synthesised by the posterior midgut were followed and it was observed that DG was the major lipid released to Lp particles. However, the majority of phospholipids were not transferred to Lp, but remained in the tissue. The phospholipids that were synthesised and accumulated in the posterior midgut were found to be associated with Rhodnius luminal contents as structural components of PMMs.
Documentos Relacionados
- Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus
- Coronavirus Particle Assembly: Primary Structure Requirements of the Membrane Protein
- Rhodnius prolixus Malpighian tubules and control of diuresis by neurohormones
- Differentiation and genetic analysis of Rhodnius prolixus and Rhodnius colombiensis by rDNA and RAPD amplification
- Membrane assembly: movement of phosphatidylserine between the cytoplasmic and outer membranes of Escherichia coli.