Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting

Gallo, Juliana Failde, Pinhata, Juliana Maira Watanabe, Chimara, Erica, Gonçalves, Maria Gisele, Fukasawa, Lucila Okuyama, Oliveira, Rosangela Siqueira de

Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting

AUTOR(ES)

Gallo, Juliana Failde, Pinhata, Juliana Maira Watanabe, Chimara, Erica, Gonçalves, Maria Gisele, Fukasawa, Lucila Okuyama, Oliveira, Rosangela Siqueira de

FONTE

Mem. Inst. Oswaldo Cruz

DATA DE PUBLICAÇÃO

01/09/2016

RESUMO

Abstract Brazil is one of the high burden countries for tuberculosis, and a rapid diagnosis is essential for effective control of the disease. In the present study, an in-house real-time polymerase chain reaction (PCR) assay targeting the mpt64 gene for identification of Mycobacterium tuberculosis complex isolates was evaluated under routine diagnosis conditions in a reference laboratory. From May 2011 to July 2012, 1,520 isolates of mycobacteria were prospectively submitted for phenotypic and/or PRA-hsp65 identification and to real-time PCR. The mpt64 real-time PCR showed 99.7% sensitivity and 96% specificity and detected 79.4% of the cases missed by phenotypic and PRA-hsp65 identification. The in-house real-time PCR assay showed high sensitivity and specificity and was successfully implemented in the routine diagnosis of tuberculosis in a reference laboratory from a high burden setting.