Perfil hematimÃtrico e identificaÃÃo da hemoglobina do bicho preguiÃa Bradypus variegatus

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

The objective of the present study was to carry out hematimetric profile and identify the types of hemoglobin (Hb) present in the blood of the sloth, Bradypus variegatus, evaluating the influence of sex over these parameters. The present study was carried out in ten sloths (6 male and 4 female), unanaesthetized, kept in a restricted area, weighting (XÂSD) 3,6Â0,8kg, and presenting a rectal temperature of 30Â1ÂC. Blood samples were drawn with a needle introduced in the medial cephalic vein of the forelimb for hematimetric studies, blood smear electrophoresis in alkaline medium, and High Performance Liquid Cromatography (HPLC). Results are presented in a descriptive manner, and to evaluate the influence of sex Mann-Whitney non-parametric test was employed (p<0,05). The hematocrit (30,4Â4%) was lower than that found in B.tridactylus and Choloepus; the red cell count (3Â0,3x106/mm3) and the quantity of hemoglobin (11Â1g/dl) was similar to that found in other species of sloths, but, lower in regards to the some mammals with high metabolic rate. The mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean concentration of corpuscular hemoglobin (MCCH) were, respectively, 104Â4fl; 40Â6pg e 38,4Â5%. Similar to those found in Choloepus genus and higher when compared to other mammals, denoting the presence of a bigger size eritrocyte and a greater content of hemoglobin when compared to other mammals. Influence of sex was found only in red blood cell counting, which showed to be higher in males (p<0,05). The Hb electrophoresis in alkaline medium, regarding human Hb as a pattern, showed the presence of Hb in position HbA1 (15%) and in position HbH (75%) and the HPLC showed five chromatographic peaks expressing the presence of five forms of Hb in the hemolysate. This hematimetric profile and the multiplicity of Hb in the blood of sloths, B.variegatus, could be related to behavioral and physiological characteristics of these animals such as sedentary lifestyle, low metabolic rate, temperature, oxygen partial pressure, besides the capacity of undergoing prolonged periods of apnea

ASSUNTO(S)

hematologia hemoglobin fisiologia bradypus hemoglobina hematology bradypus

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