Perfil da expressão genica em raizes de milho expostas ao aluminio utilizando macroarrays

AUTOR(ES)
DATA DE PUBLICAÇÃO

2002

RESUMO

Aluminum (Al) is toxic to most plants at micromolar concentrations and decreases crop productivity in large areas in the world. The first and most dramatic symptom of Al toxicity is the inhibition of root growth, which leads to a reduced root system. Consequently, sensitive plants explore a smaller soil surface. The objectives of this work were to establish the DNA arrays technology in our group and to identify Al induced genes in maize plants to get insight on the Al tolerance and toxicity mechanisms. Macroarrays were constructed on nylon membranes using sequences from the Sugarcane EST Genome Project (SUCEST). Initially, we analyzed several factors that affect the data reproducibility and sensibilify of the method, such as the amount of DNA fixed on the membrane and normalization strategies. Because of the high similarity between maize and sugarcane, we successfully hybridized macroarrays containing sugarcane ESTs with heterologous probes from maize. The changes in the transcriptome of the root apex from the Al-tolerant (Cat 100-6) and the Al-sensitive (S158717) maize lines were evaluated in response to five Al activities. A total of 151 Al-induced genes were identified, covering a wide range of functions, such as transcription factors, kinases and enzymes related to oxidative stress response. Most of the identified genes were not previously related to Al-stress response. The expression profiles obtained from these genes were clustered by hierarchical methods and self organizing maps, allowing us to elucidate many mechanisms that plants activate in response to Al. Our results also indicated that the heterologous hybridization of macroarrays is a successful strategy for transcript profiling in large scale

ASSUNTO(S)

hibridação milho aluminio

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