Perezone, from the gorgonian Pseudopterogorgia rigida, induces oxidative stress in human leukemia cells

AUTOR(ES)

Abreu, Paula A., Wilke, Diego V., Araujo, Ana J., Marinho-Filho, José Delano B., Ferreira, Elthon G., Ribeiro, Carlos Margo R., Pinheiro, Leandro S., Amorim, Juliana W., Valverde, Alessandra L., Epifanio, Rosângela A., Costa-Lotufo, Letícia V., Jimenez, Paula C.

FONTE

Rev. bras. farmacogn.

DATA DE PUBLICAÇÃO

2015-12

RESUMO

Abstract Four bisabolanes 1–4, including perezone (1) and triacetyl perezone (2), were isolated through a bioassay-guided fractionation of the extract obtained from the Caribbean gorgonian coral Pseudopterogorgia rigida collected during an expedition cruise to the Bahamas. All isolated compounds showed to be cytotoxic toward panel of four human tumor cell lines, as quantified by the MTT assay after 72 h incubation. Perezone (1), the most active one, was further analyzed, showing to be cytotoxic, but not selective, in a 12-cell line panel comprising tumor and non-tumor, as well as human and murine cells. Additionally, 1 was assayed for cytotoxicity against HL-60 leukemic cells. Pre-treatment with an acute free radical scavenger (L-NAC) before exposure of cells to perezone virtually eliminated the generation of intracellular ROS and lessened its severe cytotoxicity. The protective effect delivered by L-NAC evidences that the mechanism of perezone-induced cytotoxicity is partially associated to production of ROS and a consequent induction of oxidative stress.

Documentos Relacionados

• Rattlesnake Crotalus molossus nigrescens venom induces oxidative stress on human erythrocytes
• Acute hyperglycemia induces an oxidative stress in healthy subjects
• Acute hyperglycemia induces an oxidative stress in healthy subjects
• Aluminum Induces Oxidative Stress Genes in Arabidopsis thaliana1
• Conus vexillum venom induces oxidative stress in Ehrlich's ascites carcinoma cells: an insight into the mechanism of induction