Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library
AUTOR(ES)
Li, Jingzhi
FONTE
Biological Procedures Online
RESUMO
We have identified a peptide substrate for molecular chaperone Hsp40 Ydj1 by utilizing the combination of phage display library screening and isothemol titration calirimetry (ITC). The initial peptide substrate screening for Hsp40 Ydj1 has been carried out by utilizing a 7-mer phage display library. The peptide sequences from the bio-panning were synthesized and object to the direct affinity measurement for Hsp40 Ydj1 by isothemol titration calirimetry studies. The peptide which has the measurable affinity with Ydj1 shows enriched hydrophobic residues in the middle of the substrate fragment. The peptide substrate specificity for molecular chaperone Hsp40 has been analyzed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=521342Documentos Relacionados
- A Role for the Hsp40 Ydj1 in Repression of Basal Steroid Receptor Activity in Yeast
- The Mammalian Hsp40 ERdj3 Requires Its Hsp70 Interaction and Substrate-binding Properties to Complement Various Yeast Hsp40-dependent Functions*
- Mechanisms for regulation of Hsp70 function by Hsp40
- The Glycine-Phenylalanine-Rich Region Determines the Specificity of the Yeast Hsp40 Sis1
- The Ydj1 molecular chaperone facilitates formation of active p60v-src in yeast.