Peptide Chain Termination, VI. Purification and Site of Action of S
AUTOR(ES)
Goldstein, J.
RESUMO
Peptide chain termination is a result of at least two events: terminator codon recognition and hydrolysis of peptidyl tRNA. A protein factor S, isolated from the supernatant of Escherichia coli B, stimulates fMet release. Factor S lowers the Km for terminator trinucleotides without altering the Vmax of release and therefore acts at terminator codon recognition. The S protein differs from initiation factors, elongation factor G, several forms of elongation factor T and release factors. The importance of the 2% Tu content in purified S is unresolved.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=282921Documentos Relacionados
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