Partial suppression of the phenotype of Escherichia coli K-12 dnaG mutants by some I-like conjugative plasmids.
AUTOR(ES)
Wilkins, B M
RESUMO
Three I-like conjugative plasmids, ColIdrd1, R144drd3, and R64drd11, which are derepressed for functions involved in conjugation, were found to suppress at least partially the phenotype of temperature-sensitive dnaG mutants of Escherichia coli K-12, as judged from the kinetics of deoxyribonucleic acid synthesis at elevated temperature in newly formed and established plasmid-containing strains. In contrast, the corresponding wild-type plasmids and three F-like derepressed conjugative plasmids, F101, R100drd1, and R1drd16, all failed to suppress. Suppression is presumably caused by a different plasmid-determined function from that which promotes survival of ultraviolet-irradiated bacteria, because both the wild-type I-like plasmids and their drd mutants protected irradiated bacteria. One possible interpretation of these results is that the product of a gene carried by certain I-like plasmids can substitute for the bacterial dnaG gene product during ongoing deoxyribonucleic acid replication.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=246140Documentos Relacionados
- A DNA primase specified by I-like plasmids.
- Analogs of the dnaB gene of Escherichia coli K-12 associated with conjugative R plasmids.
- I-like R plasmids promote degradation of stable ribonucleic acid in Escherichia coli.
- dnaB gene of Escherichia coli K-12 affects superinfection inhibition between F' plasmids.
- Frameshift Suppression by thyA Mutants of ESCHERICHIA COLI K-12
