Parâmetros da ação antimicrobiana e da citotoxidade do ozônio para aplicação na Endodontia / Parameters of the antimicrobial activity and cytotoxicity of ozone for use in Endodontics

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

31/08/2011

RESUMO

This study analyzed the action of ozone as an adjunct to endodontic therapy. So (I) evaluated the effectiveness of ozonated water in microbial reducing performed on bacterial suspension of Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus aureus - in vitro study (II) tested the efficiency of ozone gas and ozonated water in reducing microbial held in contaminated root canals - ex vivo study, and (III) evaluated the biological response of fibroblasts culture in the application of ozone, according to MTT method. In the first part of the experiment the ozonated water at concentrations of 5, 20 and 40 g/mL was placed in direct contact with the bacterial suspensions separately for one minute. Then, serial dilutions were made for plating and subsequent counting of CFU/mL. In the second part, 180 teeth were inoculated with 10 L of bacterial suspensions and incubated separately for 7 days. They were then subjected to experimental groups: Group I: contamination control, Group II: rotary instrumentation combining 1%sodium hypochlorite and gel Endo-PTC, final irrigation with EDTA-T, Group III: similar protocol to Group II with final application of ozone gas in concentration of 40 g/mL, and Group IV: protocol similar to Group II with a final irrigation with ozonated water at concentration of 40 g/mL. Following each group was done microbiological collecting and serial dilution and plating for 24 hours and subsequent counting of CFU/mL. In the third part, the analysis of cell viability of gingival fibroblasts was performed by MTT method. The PBS has been ozonized at concentrations of 10, 20 and 40 g/mL and applied on the cells. Data were tabulated and analyzed statistically by Kruskal-Wallis method, complemented by Dunn\ s method (p <0.05). The first part of the study showed that E.faecalis grew in concentrations of 5 and 20 g/mL. The P. aeruginosa and S. aureus were susceptible to all three concentrations of ozonated water, it was not possible to detect CFU/mL. In the second part of the study, the Group IV was the most effective against the tested microorganisms, with statistical difference for the other groups, especially when the teeth were infected with P. aeruginosa. Finally, the third part of the study showed that ozone, at 0 hours, caused a decrease in cell viability that was reversed in the other experimental times. The concentration of 40 g/mL provided the greatest stimulus on the final evaluation period, with statistically significant difference to the control group. Given the methodologies applied, it can be concluded that (I) the concentration of 40 g/mL was most effective at reducing the number of microorganisms, (II) the protocol established for the standard chemical preparation stage associated with ozonated water at a concentration of 40 g/mL was the most effective, (III) the ozone in contact with the gingival fibroblasts, initially caused a decrease in cell viability, which was reversed in subsequent experimental times. The concentration of 40 g/mL was the one that caused a greater reduction in the number of viable cells, however, was that provided the greatest stimulus to the end of the experiment and proved to be biocompatible with the cell line tested, and (IV) on the set of experimental data, the ozonated water at a concentration of 40 g/mL , compared with the other concentrations tested, it has the best performance in terms of its biocompatibility and antimicrobial power, justifying their inclusion in the clinical arsenal in endodontic therapy.

ASSUNTO(S)

Água ozonizada endodontic ozonated water ozone ozônio tratamento endodôntico

ACESSO AO ARTIGO

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