Overproduction and nucleotide sequence of the respiratory D-lactate dehydrogenase of Escherichia coli.
AUTOR(ES)
Rule, G S
RESUMO
Recombinant DNA plasmids containing the gene for the membrane-bound D-lactate dehydrogenase (D-LDH) of Escherichia coli linked to the promoter PL from lambda were constructed. After induction, the levels of D-LDH were elevated 300-fold over that of the wild type and amounted to 35% of the total cellular protein. The nucleotide sequence of the D-LDH gene was determined and shown to agree with the amino acid composition and the amino-terminal sequence of the purified enzyme. Removal of the amino-terminal formyl-Met from D-LDH was not inhibited in cells which contained these high levels of D-LDH.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=215007Documentos Relacionados
- Oxidation of D-lactate and L-lactate by Neisseria meningitidis: purification and cloning of meningococcal D-lactate dehydrogenase.
- D-Lactate dehydrogenase of Peptostreptococcus elsdenii.
- The crystal structure of d-lactate dehydrogenase, a peripheral membrane respiratory enzyme
- Reconstitution of D-Lactate-Dependent Transport in Membrane Vesicles from a D-Lactate Dehydrogenase Mutant of Escherichia coli*
- Mechanism of autoenergized transport and nature of energy coupling for D-lactate in Escherichia coli.