Overexpression of the FAD3 desaturase gene in a mutant of Arabidopsis.
AUTOR(ES)
Shah, S
RESUMO
A mutant of Arabidopsis contained increased levels of 18:3 fatty acids and correspondingly decreased levels of 18:2. The fatty acid phenotype was strongly expressed in root and seed tissues and this observation, together with other data, suggested that the mutation leads to increased activity of the endoplasmic reticulum 18:2 desaturase encoded by the FAD3 gene. Gel-blot analysis of RNA from wild-type and mutant plants established that FAD3 transcript levels were increased 80% in the mutant relative to the wild type. Genetic analysis demonstrated a linkage between the new mutation and the fad3 locus. Linkage of the mutation to fad3 raises the possibility that the lesion is an alteration to the promoter or another regulatory region of the FAD3 gene, which results in increased transcription.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=158447Documentos Relacionados
- Two FAD3 Desaturase Genes Control the Level of Linolenic Acid in Flax Seed
- Identification of the Arabidopsis Palmitoyl-Monogalactosyldiacylglycerol Δ7-Desaturase Gene FAD5, and Effects of Plastidial Retargeting of Arabidopsis Desaturases on the fad5 Mutant Phenotype1
- Phytoene desaturase from Arabidopsis.
- A Mutation at the fad8 Locus of Arabidopsis Identifies a Second Chloroplast [omega]-3 Desaturase.
- Functional expression of the extraplastidial Arabidopsis thaliana oleate desaturase gene (FAD2) in Saccharomyces cerevisiae.