Organization of the T-cell antigen-receptor beta-chain locus in mice.

AUTOR(ES)

Lindsten, T

RESUMO

We used pulsed-field gel electrophoresis to determine the organization of the beta-chain gene of the T-cell receptor for antigen in normal and mutant inbred strains of mice. In normal mice, the variable (V)- and constant (C)- region elements of this locus span 700-800 kilobases of chromosomal DNA. All but one of the V beta gene segments analyzed lie 5' of the J beta C beta locus (J beta represents the joining region), with the closest being 280-360 kilobases away. The mutant mouse strain SJL has an internal V beta-region gene deletion that compacts the V beta region by 100-200 kilobases. Taken together with other data, these results indicate that the beta-chain locus can use either a looping-out/deletion or an inversion mechanism to appose V beta to DJ beta gene segments (D is the diversity region) and can accomplish the former (at least) over very large distances.

Documentos Relacionados

• T-cell antigen-receptor genes in autoimmune mice.
• Genomic organization of the human T-cell antigen-receptor alpha/delta locus.
• Altered antigen receptor signaling in anergic T cells from self-tolerant T-cell receptor beta-chain transgenic mice.
• Genomic organization of the mouse T-cell receptor beta-chain gene family.
• Rearrangement of T-cell receptor beta-chain genes during T-cell development.