Order of expression of genes affecting septum location during sporulation of Bacillus subtilis.

AUTOR(ES)

Dunn, G

RESUMO

The mean volumes of stationary-phase cells of wild-type and asporogenous mutants of Bacillus subtilis have been measured. Mutants blocked at stage 0 of sporulation either produced cells that had the same volume as the developing sporangium or they divided to produce cells of one-half this volume. The order of expression of the genes affected by the mutations in these strains was determined by biochemical characterization and by construction of double sporulation mutants. Mutants that produced small cells were blocked at an earlier stage of sporulation than those that produced large cells. It is suggested that the following dependent sequence must occur before the formation of the prespore spetum: (i) the initiation of sporulation, (ii) a signal to block the final central division site, and (iii) a signal to activate a polar septum site.

Documentos Relacionados

• Timing of spoII gene expression relative to septum formation during sporulation of Bacillus subtilis.
• Expression of a Bacillus megaterium sporulation-specific gene during sporulation of Bacillus subtilis.
• Requirement for peptidoglycan synthesis during sporulation of Bacillus subtilis.
• Use of immunofluorescence to visualize cell-specific gene expression during sporulation in Bacillus subtilis.
• Use of temperature-sensitive mutants to study gene expression during sporulation in Bacillus subtilis.