OPTIMIZATION OF THE OPERATING CONDITIONS FOR RHAMNOLIPID PRODUCTION USING SLAUGHTERHOUSE-GENERATED INDUSTRIAL FLOAT AS SUBSTRATE

AUTOR(ES)

Borges, W. S., Moura, A. A. O., Coutinho Filho, U., Cardoso, V. L., Resende, M. M.

FONTE

Braz. J. Chem. Eng.

DATA DE PUBLICAÇÃO

2015-06

RESUMO

AbstractBiosurfactants have a wide range of applications in emulsions, separations, and solubilization because these chemicals reduce the surface tension and viscosity of solutions. This work studied rhamnolipid production using a batch bioreactor with a working volume of 1.5 liters, Pseudomonas aeruginosa ATCC (American Type Culture Collection) 10145 strain, and the greasy effluent from the slaughter of poultry and pigs as the substrate. The main goal of this research was to evaluate the level of aeration, agitation speed and inoculum concentration using a Central Composite Design (CCD). Experimental conditions were selected using the surface response technique obtained from the CCD, and the results were validated to test the reproducibility. The following operating conditions were selected: 1.2 vvm level of aeration, 600 rpm agitation speed, and 1.0 g/L biomass inoculum concentration. Under these conditions, the following results were obtained: the rhamnose production, surface tension and emulsifying index were 5.37 g/L, 25.6 dyne/cm and 100%, respectively.

Documentos Relacionados

• Polyhydroxybutyrate production using agro-industrial residue as substrate by Bacillus sphaericus NCIM 5149
• Optimization of the operating conditions of a lab scale Aljet mill using lactose and sucrose: A technical note
• Slaughterhouse blood as a perfusate for studying myocardial function under ischemic conditions
• Optimization of process parameters for cellulase production from Bacillus sp. JS14 in solid substrate fermentation using response surface methodology
• Solid State production of manganese peroxidases using arecanut husk as substrate