Optimization of New Sample Preparation Technique for the Determination of Methadone and Codeine in Plasma Sample by GC-FID

AUTOR(ES)
FONTE

J. Braz. Chem. Soc.

DATA DE PUBLICAÇÃO

2020-03

RESUMO

A continuous sample drop flow microextraction (CSDF-ME) technique was modified for the pretreatment of plasma samples in the determination of methadone and codeine. The limit of detection and linear range for methadone were 15.0 µg L-1 and 0.1-10.0 mg L-1, respectively. The detection limit and linear range for codeine were 25.0 µg L-1 and 0.2-15.0 mg L-1, respectively. The extraction recoveries were 41.6-52.1%, and the enrichment factors ranged from 65 to 80 for codeine and methadone, respectively. Intra- and interday precisions of the technique were calculated for codeine and methadone concentrations of 0.4 mg L-1 and were 4.2-7.6% and 2.5-5.1%, respectively. Also, the accuracy (error%) of method was in the range -10 to +7.5%. The applicability of the CSDF-ME-gas chromatography-flame ionization detector (GC-FID) technique was illustrated by the determination of the mentioned drugs in human plasma. The attained relative recoveries of methadone and codeine in the 90.0-107.5% range demonstrated the excellent capability of the optimized technique for the microextraction of the mentioned drugs from plasma. The CSDF-ME-GC-FID is a rapid, repeatable and very simple technique that requires a low sample amount.

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