Optimization of alternate-strand triple helix formation at the 5"-TpA-3" and 5"-ApT-3" junctions.
AUTOR(ES)
Brodin, P
RESUMO
Alternate-strand triple helix formation was optimized at the two junction steps, the 5"-TpA-3" and 5"-ApT-3" junctions. Footprint experiments, gel retardation assays and thermal denaturation measures on a sequence appropriately designed with two adjacent alternate-strand polypurine tracts points out that the addition of an adenine residue and the removal of one nucleotide should facilitate the crossing strands at the 5"-TpA-3" junction and at the 5"-ApT-3" junction, respectively. These results provide a 'switch code' for the construction of alternate-strand triple helix forming oligonucleotides which open new possibilities for extending the range of applications of antigene strategy.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=148526Documentos Relacionados
- Nonenymatic ligation of double-helical DNA by alternate-strand triple helix formation.
- Comparison of antiparallel A.AT and T.AT triplets within an alternate strand DNA triple helix.
- Oligonucleotide-directed triple helix formation at adjacent oligopurine and oligopyrimidine DNA tracts by alternate strand recognition.
- Alternate strand recognition of double-helical DNA by (T,G)-containing oligonucleotides in the presence of a triple helix-specific ligand.
- Effect of third strand composition on the triple helix formation: purine versus pyrimidine oligodeoxynucleotides.
