Optical measurements of intracellular pH and magnesium in frog skeletal muscle fibres

AUTOR(ES)

Baylor, S. M.

RESUMO

1. Single twitch fibres were isolated from frog muscle, then mounted in a chamber which was positioned on an optical bench. The fibres were immobilized by high stretch (sarcomere spacing 3·9-4·3 μm) and by placement on a pedestal. Their optical properties were determined by illuminating a 35-65 μm diameter spot with quasimonochromatic light of intensity I0 and measuring the intensity I of the transmitted light. Since the main purpose of the experiments was to draw inferences from the absorbance spectra of different indicator dyes injected into the fibres, all results were expressed in terms of absorbance A calculated from the equation [Formula: see text]. Changes in absorbance ΔA were calculated from the differential form of the equation [Formula: see text].

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