Oligonucleotides with rapid turnover of the phosphate groups occur endogenously in eukaryotic cells.
AUTOR(ES)
Plesner, P
RESUMO
Endogenous oligonucleotides were found in trichloroacetic acid extracts of hamster lung fibroblasts and Tetrahymena cells. Peaks of radioactivity that eluted with retention times similar to oligonucleotide markers (5- to 50-mer) were found by HPLC in cells labeled briefly with 32Pi. Only minute amounts of UV-absorbing material were detected, consistent with a rapid turnover of phosphate groups. The 32P-labeled material also migrated as oligonucleotides on 20% polyacrylamide gels; it was not hydrolyzed by alkaline phosphatase but was digested by snake venom phosphodiesterase, S1 nuclease, and pancreatic RNase and was phosphorylated by T4 polynucleotide kinase. The 32P-labeled material isolated by HPLC was alkali labile and the hydrolyzate ran as nucleotides on paper chromatography. It is concluded that the oligonucleotides are mainly oligoribonucleotides, but it is possible that oligodeoxynucleotides are also present.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=304556Documentos Relacionados
- Investigation of activation of phosphate groups in mono- and oligonucleotides with mesitoyl chloride.
- Enhanced translation and increased turnover of c-myc proteins occur during differentiation of murine erythroleukemia cells.
- Rapid turnover of mannitol-1-phosphate in Escherichia coli.
- Rapid turnover of acetyl groups in the four core histones of simian virus 40 minichromosomes.
- The turnover of tRNAs microinjected into animal cells.