O2A progenitor cells transplanted into the neonatal rat brain develop into oligodendrocytes but not astrocytes.
AUTOR(ES)
Espinosa de los Monteros, A
RESUMO
The differentiation of the bipotential O2A progenitor cell into an oligodendrocyte or a type 2 astrocyte has been well documented in cell cultures of various regions of the central nervous system. The appropriate tools to prove its existence in vivo have been lacking. We report on an in vitro-in vivo approach that combines stable labeling of an enriched population of cultured O2A progenitors by the fluorescent dye fast blue, followed by their transplantation into neonatal rat brains, which allowed us to study the influence of the brain microenvironment on their lineage decision. The grafted cells survived well and 21 days after grafting nearly all were positive for the oligodendroglial marker galactocerebroside. Surprisingly, the fast blue-positive grafted cells did not stain for the astroglial marker glial fibrillary acidic protein. These results indicate that the O2A progenitor's plasticity is restricted by the in vivo environment, resulting in the developmental exclusion of the type 2 astrocyte initially described in vitro.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=45597Documentos Relacionados
- A rapid silver impregnation technique for oligodendrocytes, microglia, and astrocytes.
- Histamine induces oscillations of mitochondrial free Ca2+ concentration in single cultured rat brain astrocytes.
- O2 deprivation induces a major depolarization in brain stem neurons in the adult but not in the neonatal rat.
- Albumin stimulates uptake of calcium into subcellular stores in rat cortical astrocytes.
- Glutamate receptors activate Ca2+ mobilization and Ca2+ influx into astrocytes.