Nucleotide sequences of murine intracisternal A-particle gene LTRs have extensive variability within the R region.
AUTOR(ES)
Christy, R J
RESUMO
Nucleotide sequences of the long terminal repeats (LTRs) of four murine intracisternal A-particle (IAP) genes IAP62, 19, 81 and 14 were determined. Each IAP LTR contains three sequence domains, 5'-U3-R-U5-3', and each is bound by 4 bp imperfect inverted repeats. The transcriptional regulatory sequences, CAAT and TATA, as well as the enhancer core sequence GTGGTAA are conserved and precisely positioned within the U3 region. In the R region, the sequence AATAAA is located twenty base pairs preceding the dinucleotide CA, the polyadenylation site. In IAP19 and IAP81, the 5' and 3' LTRs are flanked by a six nucleotide direct repeat of cellular sequences representing the possible integration sites for these IAP proviruses. Both the size and sequences of different IAP LTRs vary considerably, with the majority of the variation localized within the R regions. The size of R varies from 66 bp in IAP14 to 222 bp in IAP62; in contrast, the U3 and U5 regions are all similar in size. These extra sequences within the R region of large LTRs consist of several unusual directly repeating sequences which account for this variability.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=340991Documentos Relacionados
- Terminally redundant sequences in cellular intracisternal A-particle genes.
- Nucleotide sequence of the Chinese hamster intracisternal A-particle genomic region corresponding to 5'LTR-GAG.
- Intracisternal A-particle gene expression in normal mouse thymus tissue: gene products and strain-related variability.
- Structural analysis of type II variants within the mouse intracisternal A-particle sequence family.
- Stage-specific expression of intracisternal A-particle sequences in murine myelomonocytic leukemia cell lines and normal myelomonocytic differentiation.
