Nucleotide sequence of the genes III, VI and I of bacteriophage M13.

AUTOR(ES)

van Wezenbeek, P

RESUMO

A DNA region of 2750 base pairs encompassing the genes III, VI and I of bacteriophage M13 has been sequenced by the Maxam-Gilbert procedure. By establishing the nucleotide changes introduced by several amber mutations, the coding region and the regulatory signals of each gene have been deduced. The genes appear to span 1275 base pairs (gene III; mol.wt. 44,748) 339 base pairs (gene VI; mol.wt. 12,264) and 1047 base pairs (gene I; mol.wt. 39,500). Their separating non-codogenic regions are extremely short, namely two and one base pair, respectively. The C-terminal end of gene I, however, intrudes 23 nucleotides into gene IV. From the nucleotide sequence it appears that the minor capsid protein of the phage, which is encoded by gene III, is synthesized in a precursor form containing 18 extra amino acids at its N-terminal end. Furthermore, in this capsid protein two clusters of a fourfold repeat of the sequence Glu-Gly-Gly-Gly-Ser are apparent. Gene VI appears to code for a small, extremely hydrophobic polypeptide. Its total hydrophobic amino acids content of 51% suggests that this protein can only function in the host cell membrane.

Documentos Relacionados

• Transcription of bacteriophage M13 DNA: existence of promoters directly preceding genes III, VI, and I.
• Nucleotide sequence of gene VII and of a hypothetical gene (IX) in bacteriophage M13.
• Illegitimate recombination at the replication origin of bacteriophage M13.
• Initiation and termination signals for transcription in bacteriophage M13.
• Oligonucleotide-directed mutagenesis of gene IX of bacteriophage M13.