Nucleotide sequence of katF of Escherichia coli suggests KatF protein is a novel sigma transcription factor.
AUTOR(ES)
Mulvey, M R
RESUMO
The katF gene of Escherichia coli has been sequenced revealing a 1086 base pair open reading frame from which the sequence of a 362 amino acid protein has been deduced. The direction of transcription of katF was confirmed by expression of the gene cloned in both directions behind a T7 promoter. The KatF protein expressed in vitro migrates with an apparent size of 42 kDa. Comparison of the katF sequence to the sequence of rpoD, which encodes the sigma subunit of RNA polymerase, revealed a 181 bp region with 65% homology and a 38 bp segment that was 87% homologous. A 62 amino acid region of the predicted KatF protein sequence was found to be 85% homologous to the corresponding sequence of sigma 70, including a segment implicated in core polymerase binding. Homology was also observed with the heat shock regulatory protein encoded by htpR.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=335226Documentos Relacionados
- DNA base sequence variability in katF (putative sigma factor) gene of Escherichia coli.
- KatF (sigma S) synthesis in Escherichia coli is subject to posttranscriptional regulation.
- Regulation of transcription of katE and katF in Escherichia coli.
- The putative sigma factor KatF is regulated posttranscriptionally during carbon starvation.
- The putative sigma factor KatF has a central role in development of starvation-mediated general resistance in Escherichia coli.