Nucleotide sequence of ilvGEDA operon attenuator region of Escherichia coli.

AUTOR(ES)

Nargang, F E

RESUMO

The nucleotide sequence of the DNA thought to contain the control region for the ilvGEDA operon in Escherichia coli has been determined by the Maxam-Gilbert procedure. The sequence includes a region that, upon transcription, would yield a leader transcript specifying a peptide 32 residues long. This putative peptide would contain four leucine, five isoleucine, and six valine residues. A model is proposed that correlates the multivalent control of the ilvGEDA operon with the extent to which this leader transcript is translated. In vitro transcription experiments yielded a transcript of about 183 nucelotides, compatible with the predictions of the model.

Documentos Relacionados

• Multivalent translational control of transcription termination at attenuator of ilvGEDA operon of Escherichia coli K-12.
• The DNA sequence of the promoter-attenuator of the ilvGEDA operon of Salmonella typhimurium.
• The nucleotide sequence preceding and including the beginning of the ilvE gene of the ilvGEDA operon of Escherichia coli K12.
• Physical analysis of deletion mutations in the ilvGEDA operon of Escherichia coli K-12.
• Regulation of ilvEDA Expression Occurs Upstream of ilvG in Escherichia coli: Additional Evidence for an ilvGEDA Operon