Nucleosome cores reconstituted from poly (dA-dT) and the octamer of histones.

AUTOR(ES)

Rhodes, D

RESUMO

In this paper we describe a detailed investigation of the reconstitution of nucleosome cores from poly (dA-dT) and the octamer of histones. We also attempted the reconstitution from the copolymers poly dA.poly dT, poly dG.poly dC and poly (dG-dC). The repeat of the reconstituted chromatin fibre is discussed. The micrococcal nuclease released poly (dA-dT) core particle is found to contain a considerably narrower DNA size distribution that of the native random DNA nucleosome core (12). In addition we have succeeded in obtaining small crystals of the poly (dA-dT) nucleosome core. The DNAase I digestion pattern of the poly (dA-dT) containing nucleosome core is presented. The periodicity of DNAase I cutting sites is found to be about 10.5 bases and is similar to that of the native nucleosome core (12, 13).

Documentos Relacionados

• Conformation of DNA in chromatin reconstituted from poly [d(A-T)] and the core histones.
• The molecular electrostatic potential and steric accessibility of poly (dA-dT). poly (dA-dT) in various conformations: B-DNA, D-DNA and 'alternating-B' DNA.
• Nuclear magnetic resonance studies of the helix-coil transition of poly (dA-dT) in aqueous solution.
• A study on the unprimed poly (dA-dT) synthesis catalyzed by preparations of E. coli DNA polymerase I.
• Conformation of 145 base pair length poly (dG-dC) . poly (dG-dC) in solution and in association with histones.