Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage

AUTOR(ES)

Raderschall, Elke

FONTE

The National Academy of Sciences

RESUMO

A sensitive and rapid in situ method was developed to visualize sites of single-stranded (ss) DNA in cultured cells and in experimental test animals. Anti-bromodeoxyuridine antibody recognizes the halogenated base analog incorporated into chromosomal DNA only when substituted DNA is in the single strand form. After treatment of cells with DNA-damaging agents or γ irradiation, ssDNA molecules form nuclear foci in a dose-dependent manner within 60 min. The mammalian recombination protein Rad51 and the replication protein A then accumulate at sites of ssDNA and form foci, suggesting that these are sites of recombinational DNA repair.

Documentos Relacionados

• Targeted recombination with single-stranded DNA vectors in mammalian cells.
• Mammalian single-stranded DNA binding proteins and heterogeneous nuclear RNA proteins have common antigenic determinants.
• Transfection and homologous recombination involving single-stranded DNA substrates in mammalian cells and nuclear extracts.
• Single-stranded regions on unintegrated avian retrovirus DNA.
• The origin of nascent single-stranded DNA extracted from mammalian cells.