Novel method to extract large amounts of bacteriocins from lactic acid bacteria.
AUTOR(ES)
Yang, R
RESUMO
Antimicrobial peptides, bacteriocins, produced by lactic acid bacteria were adsorbed on the cells of producing strains and other gram-positive bacteria. pH was a crucial factor in determining the degree of adsorption of these peptides onto cell surfaces. In general, between 93 and 100% of the bacteriocin molecules were adsorbed at pHs near 6.0, and the lowest (< or = 5%) adsorption took place at pH 1.5 to 2.0. On the basis of this property, a novel isolation method was developed for bacteriocins from four genera of lactic acid bacteria. By using this method we made preparations of pediocin AcH, nisin, sakacin A, and leuconocin Lcm1 that were potent and concentrated. This method produced a higher yield than isolation procedures, which rely on precipitation of the bacteriocins from the cell-free culture liquor. It is simple and can be used to produce large quantities of bacteriocins from lactic acid bacteria to be used as food biopreservatives.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=183103Documentos Relacionados
- Common Mechanistic Action of Bacteriocins from Lactic Acid Bacteria †
- Bacteriocins from lactic acid bacteria: purification, properties and use as biopreservatives
- Comparative Studies of Class IIa Bacteriocins of Lactic Acid Bacteria
- Inhibition of food-borne bacterial pathogens by bacteriocins from lactic acid bacteria isolated from meat.
- Strong Synergy between a Eukaryotic Antimicrobial Peptide and Bacteriocins from Lactic Acid Bacteria