Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA.

AUTOR(ES)

Allen, R D

RESUMO

We developed a rapid, sensitive, and specific PCR-based assay for human cytomegalovirus (HCMV). The assay includes primer and probe sequences derived from conserved HCMV nucleotide sequences and nonradioactive hybridization-confirmation. The assay detected between 10 and 100 viral genomes. All HCMV clinical isolates tested (39 of 39) gave positive reactions.

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