Nonpermissivity of human peripheral blood lymphocytes to adenovirus type 2 infection.
AUTOR(ES)
Horvath, J
RESUMO
The capacity of freshly explanted human peripheral blood lymphocytes (PBL) to support the replication of human adenovirus type 2 (Ad2) was investigated. Unlike other types of human cells, PBL were found to be highly nonpermissive. Ad2 adsorbed 30 to 40% of both T and non-T cells. Virus uncoating was very slow and inefficient, resulting in a 40-fold reduction compared with HEp-2 cells. On a population basis, viral DNA synthesis was reduced 460-fold and infectious virus production was reduced 10(6)-fold. Only 0.35% of PBL produced infectious centers, yielding 0.8 PFU per infected cell. Phytohemagglutinin stimulation increased DNA synthesis 23-fold, infectious centers 11-fold, and virus yield 14-fold. We conclude that resting human PBL are highly nonpermissive to Ad2 infection and that phytohemagglutinin can only marginally lift this nonpermissiveness.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=250535Documentos Relacionados
- Definition of a subset of human peripheral blood mononuclear cells that are permissive to human cytomegalovirus infection.
- Adenovirus type 2 nuclear RNA accumulating during productive infection.
- Response of human lymphocytes to measles virus after natural infection.
- Resistance to human immunodeficiency virus type 1 infection conferred by transduction of human peripheral blood lymphocytes with ribozyme, antisense, or polymeric trans-activation response element constructs.
- Role of adenovirus type 2 early region 4 in the early-to-late switch during productive infection.
