Noninvasive genetic sampling of endangered muriqui (Primates, Atelidae): efficiency of fecal DNA extraction
AUTOR(ES)
Chaves, Paulo B., Paes, Marcela F., Mendes, Sérgio L., Strier, Karen B., Louro, Iúri D., Fagundes, Valéria
FONTE
Genetics and Molecular Biology
DATA DE PUBLICAÇÃO
2006
RESUMO
The muriqui (Brachyteles) is one of the most endangered primates in the world, however little is known about the viability of the remaining populations. We evaluated the technique of extracting DNA from wild muriqui feces for PCR applications. In order to determine the effect of the DNA in subsequent amplifications, we analyzed five different extracts. The importance of the recommended BSA and the HotStarTaq DNA polymerase was tested. The minimal conditions to successfully amplify highly degraded fecal DNA were determined, showing that the recommended reagents are not required. We envision that this method may be useful in further conservation management studies.
Documentos Relacionados
- Filogeografia do muriqui do sul, Brachyteles arachnoides (Primates, Atelidae)
- Genetic structure in two northern muriqui populations (Brachyteles hypoxanthus, Primates, Atelidae) as inferred from fecal DNA
- Diferenças entre sexos no uso do espaço vertical por muriqui-do-norte Brachyteles hypoxanthus - Kuhl, 1820 (Primates, Atelidae)
- Três anos sobre a demografia de um grupo de Alouatta guariba clamitans Cabrera (Primates, Atelidae): crescimento e fragmentação
- Os sete primeiros meses de um infante de Alouatta guariba (Humboldt) (Primates, Atelidae): interações e desenvolvimento dos padrões comportamentais