Níveis e avidez de anticorpos IgG específicos para a porção de 19kDa da região C-terminal da proteína-1 de superfície de merozoítos de P. vivax (MSP1 19) em grupos populacionais expostos à malária / Level and avidity of specific IgG antibodies to C-terminal 19kDa of Plasmodium vivax merozoite surface protein 1 (MSP119) in population groups exposed to malaria

AUTOR(ES)

Mônica Eriko Kudó

DATA DE PUBLICAÇÃO

2007

RESUMO

The aim of this work was to study the level and avidity of IgG antibodies specific to Plasmodium vivax using the recombinant protein corresponding to 19kDa C-terminal of the merozoite surface protein 1 region (PvMSP119) in individuals living in distinct malaria endemic areas, using ELISA test. Thus, samples from individuals exposed to malaria, with patent infection or not were evaluated. The reaction conditions were standardized, yielding 95.00% sensitivity with sera from P. vivax infected patients and 99.50% specificity with sera from individuals non-exposed to P. vivax malaria. Just 7.14% of patient samples with P. falciparum malaria reacted to PvMSP119. The investigation of different groups of vivax malaria patients showed significant differences between patients with primo infection and those with past malaria episodes. The levels and avidity of IgG were lower in primo infected ones. The predominance of low avidity IgG in P. vivax primo infected patients may suggest low grade of protection even in high level antibody sera. The analysis of non-infected subjects showed a negative association of IgG levels and time elapsed since last malaria episode, and a positive association to the number of malaria episodes. For individuals who had had last malaria episode till maximum six months before the collecting of blood samples, avidity indexes showed positive association for both parameters described before. Individuals sporadically exposed to malaria transmission (Belém group) and who experienced their last episode by P. vivax had significant high levels and proportions of specific antibodies when compared to subjects continuously exposed (Alta Floresta group). Otherwise, higher avidity levels were detected in high transmission areas. In god mines and forest areas of Alta Floresta, higher IgG and avidity levels were obtained, as compared to the other regions of the municipality. No correlation was observed between anti-PvMSP119 IgG levels and avidity levels.

ASSUNTO(S)

elisa antibodies malaria population groups plasmodium vivax proteína 1 de superfície de merozoíto plasmodium vivax elisa immunoglobulin g grupos populacionais imunoglobulina g anticorpos merozoite surface protein 1 malária antibodies avidity afinidade de anticorpos

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