New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli

AUTOR(ES)

Xavier, Mauro Aparecido Souza, Kipnis, André, Torres, Fernando Araripe Gonçalves, Astofi-Filho, Spartaco

FONTE

Brazilian Journal of Microbiology

DATA DE PUBLICAÇÃO

2009-12

RESUMO

We report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857 repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli.

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